Identification of novel 1,4-dioxane degraders and related genes from activated sludge by taxonomic and functional gene sequence analysis

This study used integrated omics technologies to investigate the potential novel pathways and enzymes for 1,4-dioxane degradation by a consortium enriched from activated sludge of domestic wastewater treatment plant. An unclassified genus belonging Xanthobacteraceae increased significantly after magnetic nanoparticle-mediated isolation degraders. Species with relatively higher abundance (> 0.3%) were identified present high metabolic activities in biodegradation process through shotgun sequencing. The functional gene investigations revealed that Xanthobacter sp. 91, 126, Rhizobiales strain carried 1,4-dioxane-hydroxylating monooxygenase genes. 126 contained genes coding glycolate oxidase, which was main enzyme responsible utilization intermediates TCA cycle, further proven specific oxidase inhibitor, ?-hydroxy-2-pyridinemethanesulfonic acid. expanded detailed pathway proposed on basis three major (2-hydroxy-1,4-dioxane, ethylene glycol, oxalic acid) confirmed metabolomics. These findings microbial community function as well will be valuable predicting natural attenuation or reconstruction bacterial enhanced remediation 1,4-dioxane-contaminated sites treatment.